Journal: Brain Sciences

Article Title: Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors

doi: 10.3390/brainsci10060344

Figure Lengend Snippet: Expression of Ca V 2 high voltage activated calcium channels. ( A – C ) D50 iDNs showed robust expression of all Ca V 2 high voltage activated calcium channels in regions of the soma, neurites, as well as axonal varicosities. Arrowheads indicate individual ( A ) Ca V 2.1/PQ-type, ( B ) Ca V 2.2/N-type, and ( C ) Ca V 2.3/R-type clusters along neurite structures of iDNs. Line scan measurements for individual Ca V 2 clusters on TUJ1-IR+ varicosities revealed their location within putative synaptic structures in iDN cultures, line scan was set to 3 µm. ( D – F ) Mouse neurons (mDRG) served as controls, line scans performed on neurite segments, no axonal varicosities were detected, 2–3 individual differentiations, scale bars 20 µm (overview, soma), 10 µm (enlargement, axon), 1 µm (axonal varicosities, putative synapses).

Article Snippet: Primary antibodies used were anti-synapsin (1:1000, mouse monoclonal, Synaptic Systems (SySy); #106011 reference [ ]); anti-BRN3A (1:500 rabbit polyclonal, SySy, #411003, reference [ ]); anti-ISL-1 (1:500, rabbit polyclonal, SySy, #406003 reference [ ]); anti-Ca V 2.1 (1:1000, rabbit polyclonal, SySy, #152103 k.o verified); anti-Ca V 2.2 (1:1000, rabbit polyclonal, SySy, #152313 reference [ ]); anti-Ca V 2.3 (1:1000, rabbit polyclonal, SySy, #152403 reference [ ]); anti-RUNX1 (1:200, rabbit polyclonal, Abcam, #23980 reference [ ]); anti-p75 (1:200, rabbit polyclonal, Abcam, #AB52987 reference [ ]); anti-NKCC3 (1:500, rabbit polyclonal, Thermo Fisher Scientific #PA5-56975); anti-TRPV1 (1:200, rabbit polyclonal, Alomone Labs, #ACC-030 reference [ ]); anti-TUJ1 (1:600, mouse monoclonal, R&D Systems, #MAB1195 reference [ ]); anti-GABA A R (β2,3 chain, representing the most abundant subunit chains of GABA A Rs) (1:1000, mouse monoclonal, Chemicon, #MAB341 reference [ ]); and anti-HNC1–4 (1:600, Alomone Labs, HCN1 #APC-056, HCN2 #APC-030, HCN3 #APC-057, and HCN4 #APC-052, reference [ ]).

Techniques: Expressing

Journal: Brain Sciences

Article Title: Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors

doi: 10.3390/brainsci10060344

Figure Lengend Snippet: Summary of the used markers, level of expression, and morphological abundance in iDNs and mouse sensory neurons.

Article Snippet: Primary antibodies used were anti-synapsin (1:1000, mouse monoclonal, Synaptic Systems (SySy); #106011 reference [ ]); anti-BRN3A (1:500 rabbit polyclonal, SySy, #411003, reference [ ]); anti-ISL-1 (1:500, rabbit polyclonal, SySy, #406003 reference [ ]); anti-Ca V 2.1 (1:1000, rabbit polyclonal, SySy, #152103 k.o verified); anti-Ca V 2.2 (1:1000, rabbit polyclonal, SySy, #152313 reference [ ]); anti-Ca V 2.3 (1:1000, rabbit polyclonal, SySy, #152403 reference [ ]); anti-RUNX1 (1:200, rabbit polyclonal, Abcam, #23980 reference [ ]); anti-p75 (1:200, rabbit polyclonal, Abcam, #AB52987 reference [ ]); anti-NKCC3 (1:500, rabbit polyclonal, Thermo Fisher Scientific #PA5-56975); anti-TRPV1 (1:200, rabbit polyclonal, Alomone Labs, #ACC-030 reference [ ]); anti-TUJ1 (1:600, mouse monoclonal, R&D Systems, #MAB1195 reference [ ]); anti-GABA A R (β2,3 chain, representing the most abundant subunit chains of GABA A Rs) (1:1000, mouse monoclonal, Chemicon, #MAB341 reference [ ]); and anti-HNC1–4 (1:600, Alomone Labs, HCN1 #APC-056, HCN2 #APC-030, HCN3 #APC-057, and HCN4 #APC-052, reference [ ]).

Techniques: Expressing